Isoform-specific expression patterns of the MdPR10 gene in apple cultivars with Golden Delicious pedigree
DOI:
https://doi.org/10.59463/d79ecq54Keywords:
Malus domestica Borkh., variability, pathogen-related proteinAbstract
The Mal d 1 protein, a member of the PR-10 protein family encoded by the MdPR10 gene in Malus domestica Borkh., responds to biotic and abiotic stress stimuli. It may accumulate in plant tissues and is known to trigger allergic reactions in humans. Empirical observations show that the same individual may exhibit varying allergic responses depending on the apple cultivar consumed. The underlying cause is still not fully understood, although several hypotheses are being considered, and it is likely to be multifactorial.
In this study, the expression of eight MdPR10 isoforms (1.01, 1.02, 1.03, 1.06, 1.07, 1.08, 1.11, and 1.13) was measured across twelve M. domestica cultivars, all sharing Golden Delicious as a maternal pedigree. Isoforms 1.01 and 1.02 were consistently highly expressed with low variability, while isoforms 1.03, 1.06, 1.07, and 1.08 showed high expression variability among cultivars. Freyberg exhibited the closest expression profile to Golden Delicious, followed by Sirius and Shalimar; in contrast, Mutsu, Spencer, and Delbarestivale showed the greatest divergence.
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