The usage of the SCoT molecular markers for determining the genetical purity of the commercial hybrids of Hellianthus annuus
Keywords:
Sunflower Hybrids, Genetic Purity, Molecular Markers, PCR, SCoT primers;Abstract
Genetic purity is an important factor in the production of hybrid seeds, as it is necessary to conserve and maintain its agronomic qualities. Poor purity leads to degradation over time in sunflower hybrids, particularly in inbred lines from breeding programs.
In the present study, we investigated the use of two types of SCoT (Start Codon Targeted Polymorphism) primers for identifying genetic purity. The study included the SCoT34 and SCoT7 primers to assess the genetic purity of hybrid AH1023. Statistical calculations showed a higher polymorphism identity for the SCoT34 primer (73%) compared to the SCoT7 primer (50%). Furthermore, the number of alleles generated was 11 for the SCoT34 primer and only 4 alleles for the SCoT7 primer.
Analysis of the Jaccard coefficient of hybrid AH1023 identified 10 genotypes with common similarity and 5 genotypes that differed substantially from the group genotypically, resulting in a genetic purity of 67% for hybrid AH1023.
Thus, the SCoT primers successfully identified the initial degradation of genetic purity and provided insights into the genetic profile of hybrid AH1023.
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