Developing an "in vitro" propagation protocol for Silphium perfoliatum
DOI:
https://doi.org/10.59463/vyd0ex58Keywords:
cup plant, micropropagation, sterilization, seeds, seedlingAbstract
The Silphium L. species are indigenous to the fields, prairies, and forests of eastern and central North America. The genus Silphium (L.) belongs to the family Asteraceae, order Heliantheae. In Europe, Silphium perfoliatum was introduced in the 18th century and was initially utilized as an ornamental plant in gardens and parks in Germany, France, Switzerland, and the United Kingdom.
Micropropagation represents an alternative means of propagation that can be employed for the mass multiplication of plants in a relatively shorter time frame. The objective of this study was to develop and optimize a protocol that can be readily implemented in field culture.
The study demonstrates that the sterilization of seeds with a CuSO₄ solution, followed by their immersion in a phenolic biostimulant solution prior to placement on a simple MS culture medium, yielded the most optimal results in the production of Silphium perfoliatum explants.
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