Determination of the antioxidant activity of Oyster mushrooms from U.P. VIII RĂCHITA
DOI:
https://doi.org/10.59463/gnj7tk11Keywords:
phenolic content, flavonoid content; antioxidant activity, oyster mushrooms.Abstract
Oyster mushrooms (Pleurotus ostreatus Jacq. P. Kumm.) from forest of UP VIII Răchita were investigated for determination of polyphenol content (Folin-Ciocâlteu method), determination of total flavonoids (sodium nitrite method) and evaluation of antioxidant activity using the DPPH method.
The highest total polyphenol value was obtained for the methanol extract of Pleurotus ostreatus subjected to the ultrasound procedure, respectively 14.74 ± 0.20 μgGAE/g, followed by the ethanol extract obtained by shaking (14.62 ± 0.04 μgGAE/g) and ultrasound (14.50 ± 0.09 μgGAE/g). Whilst the lowest value, 13.90 ± 0, 03 μgGAE/g was indicated by the methanol extract obtained by shaking.
The minimum value was indicated by the extract of Pleurotus ostreatus in methanol obtained by ultrasound, this being followed by the same methanol extract, nonetheless obtained by shaking with a value of 133.77 ± 0.57 μg/ml.
The highest percentage of inhibition was recorded for the methanol extract of Pleurotus ostreatus subjected to the stirring technique, having a value of 29.69%. This was followed by the methanol extract obtained by ultrasound with a PI of 25.65%. For the other two ethanol extracts, the percentages did not exceed the value of 20%, respectively 18.78% for the extract in ethanol obtained by stirring and 17.09% for the one obtained by ultrasound.
References
Al-Matani, S.K., Ruqaiya, N.S. Al-Wahaibi, Mohammed, A.H. (2015), Total Flavonoids Content and Antimicrobial Activity of Crude Extract from Leaves of Ficus Sycomorus Native to Sultanate of Oman, Karbala International Journal of Modern Science 1 (3): 166–71
Aryal, S., Manoj, K.B., Krisha, D., Puspa, K., Roshani, G., Niranjan, K. (2019), Total Phenolic Content, Flavonoid Content and Antioxidant Potential of Wild Vegetables from Western Nepal, Plants 8 (4)
Bhatia, P., Aureli, F., DʹAmato, M., Prakash, R., Cameatra, S.S., Nagaraja, T.P., Cubadda, F. (2013), Selenium bioaccessibility and speciation in biofortified Pleurotus mushrooms growth on selenium-rich agricultural residues, Food Chem 140:225–230
Bhatia, P., Prakash, R., Prakash, N.T. (2014), Enhanced antioxidant properties as a function of selenium uptake by edible mushrooms cultivated on selenium-accumulated waste post-harvest wheat and paddy residues, Int J Recycl Org Waste Agric 3:127–132
Côté, J., Caillet, S., Doyon, G., Sylvain, J.F., Lacroix, M. (2010), Bioactive compounds in cranberries and their biological properties,Crit Rev Food Sci Nutr 50:666–679
Chen, L.Y., Cheng, C.W., Liang, J.Y. (2015), Effect of esterifica- tion condensation on the Folin–Ciocalteu method for the quantitative measurement of total phenols, Food Chemistry, vol. 170, pp. 10–15, citat de Carmona-Hernandez et al., 2021
Estrada, A.E.R., Lee, H.J., Beelman, R.B., del Mar Jimenez-Gasco, M., Royse, D.J. (2009), Enhancement of the antioxidants ergothioneine and selenium in Pleurotus eryngii var. eryngii basidiomata through cultural practices, World J Microbiol Biotechnol 25:1597–1607
Michalak, A. (2006), Phenolic compounds and their antioxidant activity in plants growing under heavy metal stress, Pol J Environ Stud 15:523–530
Newell, A.M., Yousef, G.G., Lila, M.A., Ramírez-Mares, M.V., de Mejia, E.G. (2010), Comparative in vitro bioactivities of tea extracts from six species of Ardisia and their effect on growth inhibition of HepG2 cells, J Ethnopharmacol 130:536–544
Pękal, A., Pyrzynska, K. (2014), Evaluation of Aluminium Complexation Reaction for Flavonoid Content Assay, Food Analytical Methods 7 (9): 1776–82
Rusak, G., Komes, D., Likić, S., Horžić, D., Kovač, M. (2008), Phenolic content and antioxidative capacity of green and white tea extracts depending on extraction conditions and the solvent used, Food Chemistry, vol. 110, no. 4, pp. 852–858, 2008, in Carmona-Hernandez et al., 2021
Sánchez-Rangel, J.C., Benavides, J., Heredia, J.B., Cisneros-Zevallos, L., Jacobo-Velázquez, D.A. (2013), The Folin–Ciocalteu assay revisited: improvement of its specificity for total phenolic content determination, Analytical Methods, vol. 5, no. 21, pp. 5990–5999, in Carmona-Hernandez et al., 2021
Sies, H. (2007), Total antioxidant capacity: appraisal of a concept, The Journal of nutrition 137.6:1493-1495
Tudor, I. (2010), Mushrooms in Romania's wild flora, Ed. Gramen, București
*** Ocolul Silvic Teregova - Amenajamentul U.P. VIII Rachita
