In vitro culture of Grasa de Cotnari cv. and evaluation of the genetic fidelity using SCoT markers
DOI:
https://doi.org/10.59463/qskg2w78Keywords:
micropropagation, PGRs, Start Codon Targeted markers, Vitis vinifera L.Abstract
In this study, eight variants (V1-V8) of Murashige and Skoog (MS) medium were utilized, supplemented with cytokinins such as kinetin (Kin), meta-topolin (mT), and 6-benzyladenine (BA), along with indole-3-butyric acid (IBA) as an auxin source: MS+0.5 mg/L Kin (V1), MS+0.75 mg/L Kin (V2), MS+0.5 mg/L mT (V3), MS+1 mg/L mT (V4), MS+0.2 mg/L BA (V5), MS+0.5 mg/L BA (V6), MS+0.2 mg/L BA+0.2 mg/L IBA (V7), and MS+0.5 mg/L BA+0.2 mg/L IBA (V8). The results indicated that the presence of Kin and mT led to the formation of the most elongated shoots in the following MS medium variants: 4.98 cm (V3), 4.81 cm (V2), 4.74 cm (V1), and 4.72 cm (V4), although there were no statistically significant differences among them. The highest number of shoots per explant was achieved on the MS+0.5 mg/L BA+0.2 mg/L IBA (V8) medium with 7.33 shoots. A genetic fidelity assessment of the regenerated plants was conducted using Start Codon Targeted (SCoT) markers, which confirmed their genetic stability. Optimizing the micropropagation protocol for Grasă de Cotnari is crucial for producing uniform and high-quality planting material.
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