In vitro propagation and genetic stability assessment of Plectranthus amboinicus and Thymus × citriodorus
DOI:
https://doi.org/10.59463/wceqpw25Keywords:
aromatic plants, micropropagation, Start Codon Targeted markersAbstract
This study aimed to investigate the genetic stability and uniformity of Plectranthus amboinicus (Lour.) and Thymus × citriodorus (Pers.) Schreb. propagated in vitro for 16 months using Start Codon Targeted (SCoT) molecular markers. Additionally, several growth parameters were assessed, including the number of shoots per explant, shoot length, in vitro rooting percentage, and root and shoot length of rooted plantlets. T. citriodorus exhibited a higher number of shoots per explant (29.8 ± 1.26) compared to P. amboinicus (15.6 ± 1.22). However, the in vitro rooting percentage was higher in P. amboinicus (96.67%) than in T. citriodorus (71.67%). Rooted shoots were acclimatized in perlite for 30 days, achieving a 100% survival rate for both species. No polymorphism was detected, confirming the high clonal fidelity of the regenerated plants.
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