The influence of hormonal balance in the multiplication of two varieties of Hylocereus spp.

Authors

  • Marcel DANCI University of Life Sciences "King Mihai I" from Timisoara Author
  • Simion ALDA University of Life Sciences "King Mihai I" from Timisoara Author
  • Teodor CRISTEA University of Life Sciences "King Mihai I" from Timisoara Author
  • Ioan DAVID University of Life Sciences "King Mihai I" from Timisoara Author
  • Madalina VULPE Politehnica Univeristy of Bucharest Author
  • Felicia PETCU University of Life Sciences "King Mihai I" from Timisoara Author
  • Liana ALDA University of Life Sciences "King Mihai I" from Timisoara Author
  • Alexandra BECHERESCU University of Life Sciences "King Mihai I" from Timisoara Author
  • Anca DRAGUNESCU University of Life Sciences "King Mihai I" from Timisoara Author

DOI:

https://doi.org/10.59463/g8chbn26

Keywords:

pitaya; micropropagation; Murashige and Skoog; growth regulators, culture media, in vitro culture

Abstract

Dragon fruit (Hylocereus undatus) belongs to the Cactaceae family and is a species cultivated for fruit consumption due to its rich content of vitamins, minerals and an excellent source of antioxidants (betacyanin), but also vegetable albumin, vitamins and water-soluble fibres. The best method for rapid plant multiplication is cell and tissue culture, so in this study we followed the influence of different growth hormones on micropropagation and rooting, in two varieties of pitaya (Vietnamese White dragon fruit and Red Jiana Dragon Fruit). Thus, the shoots obtained from the seeds were grown on Murashige and Skoog (MS) culture medium supplemented with two types of cytokinins: 6-benzyl amino purine (BAP) – 2.5 mg/l and kinetin (KIN) – 2 mg/ l, respectively naphthaleneacetic acid (NAA) – 1 mg/l, for multiplication, and for the rooting of the shoots, Murashige and Skoog (MS) medium supplemented with 1 mg/l IBA, respectively the medium without growth regulators, was used. The results obtained were different depending on the variety, but also on the type of growth regulators used.

References

Bello-Bello, J. J., Schettino-Salomón, S., Ortega-Espinoza, J., & Spinoso-Castillo, J. L. (2021), A temporary immersion system for mass micropropagation of pitahaya (Hylocereus undatus). In Vitro Cellular & Developmental Biology – Plant, 57(4), 519-528.

Chen, J.T., Chen, Z.Y., & Chang, W.C. (2016), In vitro propagation and regeneration of cacti and succulents: advances and applications. Plant Cell, Tissue and Organ Culture, 126(1), 1–15.

Esquivel, P., Stintzing, F.C., & Carle, R. (2012), Phenolic compound profiles and betacyanin content of pitaya (Hylocereus spp.) fruits. Food Research International, 46(1), 258–263.

Hernández, Y., Ramírez-Mosqueda, M.A., & Cruz-Sosa, F. (2014), In vitro morphogenesis of Hylocereus undatus: influence of cytokinins and auxins on shoot and root formation. Biologia Plantarum, 58(2), 327–334.

Ismail, A. M. (2023), Clonal micropropagation of purplish-red dragon fruit (Hylocereus costaricensis) newly introduced to Egypt. Al-Azhar Journal of Basic & Applied Sciences, (2023), Article 259210.

Lee, Y.-C., & Chang, J.-C. (2022), Development of an improved micropropagation protocol for red-fleshed pitaya ‘Da Hong’ with and without activated charcoal and plant growth regulator combinations. Horticulturae, 8(2), 104.

Lema-Rumińska, J., & Kulus, D. (2014), Micropropagation of ornamental cacti—current status and prospects. Prop. Ornamental Plants, 14(2), 71–83.

Ramírez-Mosqueda, M.A., Hernández-Ramírez, Y., & De-la-Peña, C. (2019), Micropropagation and acclimatization of Hylocereus species: effects of cytokinins and auxins on shoot proliferation and rooting. Scientia Horticulturae, 256, 108613.

Singh, K. K., & Rani, D. (2023), Recent advances and prospects for dragon fruit (Hylocereus spp.) plant propagation techniques. International Journal of Environment and Climate Change, 13(11), 568-573.

Silva, T.L., Oliveira, R.P., & Costa, J.L. (2018), Germination and in vitro establishment of Hylocereus spp. seeds on different culture media. Revista Brasileira de Fruticultura, 40(2), e-411.

Suman, K., Roja Rani, A., & Veera Reddy, P. (2017), Response of dragon fruit (Hylocereus undatus) explants on MS media with growth regulators under in vitro for mass multiplication. Agriculture Update, 12(TechSear-9), 2371-2375.

Tenore, G.C., Novellino, E., & Basile, A. (2019), Nutraceutical and antioxidant properties of dragon fruit (Hylocereus spp.) and their potential health benefits. Journal of Food Biochemistry, 43(2), e12761.

Mizrahi, Y., & Nerd, A. (1999), Climbing and columnar cacti: new arid land fruit crops. Perspectives in New Crops and New Uses, 358–366.

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Published

2025-12-18

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Articles