Direct organogenesis and molecular characterization of Saintpaulia ionantha regenerants via SCoT markers
DOI:
https://doi.org/10.59463/bd5jah56Keywords:
tissue culture, plant regeneration, genetic stability, molecular markers; SCoTAbstract
The African violet (Saintpaulia ionantha H. Wendl.) is a valuable ornamental species widely used as a model plant in biotechnology due to its high regenerative capacity and adaptability to in vitro culture conditions. This study aimed to evaluate the regeneration and multiplication potential of S. ionantha via direct organogenesis and to assess the genetic stability of regenerated plantlets using SCoT (Start Codon Targeted) molecular markers. Micropropagation was performed on Murashige–Skoog (MS) medium supplemented with three hormonal combinations: V1 – 2 mg/l BA, V2 – 2 mg/l BA + 0.5 mg/l NAA, and V3 – 2 mg/l BA + 1 mg/l NAA. Leaf explants were cultured under controlled environmental conditions, and regenerated fragments from ten plantlets were subjected to genomic DNA extraction and SCoT marker analysis (primers T4, T5, T7). The results demonstrated a high efficiency of direct organogenesis, with variant V3 yielding the highest number of regenerants and greatest shoot height. Molecular analysis revealed no detectable polymorphisms among regenerants, indicating strong genetic stability and minimal somaclonal variation. Overall, the study confirms the suitability of direct organogenesis for efficient micropropagation of S. ionantha and highlights SCoT markers as reliable tools for evaluating clonal fidelity.
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