Optimizing in vitro regeneration and micropropagation of raspberry genotypes
Keywords:
raspberry plants, in vitro culture, hormonal balanceAbstract
This study investigates the in vitro regeneration and multiplication of three raspberry genotypes: RUVI, OPAL, and HERITAGE. Utilizing optimized Murashige-Skoog (MS) culture media enriched with varying concentrations of cytokinin BAP and gibberellin GA3, the research identifies hormonal variant V3 (0.5 mg/L BAP, 0.5 mg/L GA3) as the most effective for promoting shoot regeneration and multiplication. RUVI demonstrated the highest regeneration rate (60%) and superior rooting and acclimatization efficiencies (90% each), emphasizing its robustness and adaptability. HERITAGE showed moderate performance, while OPAL displayed the lowest rooting rate (60%), highlighting variability among genotypes. The study further underscores the critical role of acclimatization, where controlled environmental conditions ensured a smooth transition of regenerants from sterile in vitro setups to natural growth environments. These findings provide actionable insights for optimizing micropropagation techniques, enhancing the scalability and efficiency of raspberry cultivation programs.
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